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B2616 - Calorimetric investigation of rat myometrium as a function of gestational age
The aim of the present work was the characterization of the myometrial samples of healthy control and chronic inflammation-exposed rats as a function of gestational stage by means of differential scanning calorimetry (DSC). Non-pregnant, mid-pregnant (day 14) and late-pregnant (day 21) animals were included. The spontaneous, KCl-stimulated as well as maximal motor activity was additionally recorded in vitro. Calorimetric enthalpy (?H) normalised on wet mass significantly increased as pregnancy progressed in case of non-inflamed uteri. Inflammation resulted in a significant decrease in ?H/m of samples from non-pregnant till pregnant rats. Maximal contractility increased by inflammation in all tested stages of gestation. Our present results indicate that DSC can be considered as a useful tool for the investigation of the uterine function modified by gestation or a pathological condition.
Istvn Zupk--, Gbor Cs'k, George Falkay, Gza Regdon Jr, Dnes L?rinczy, Thermochimica Acta, 496 (2009) 26-29
B2615 - DSC, as a new method to verify the exact warm and cold ischemic injury during small bowel surgery
The fact that small bowel is extremely sensitive to ischemia/reperfusion injury had encouraged us to compare the influences of warm and cold ischemia on the intestinal structural changes by differential scanning calorimetry (DSC) method. Warm and cold ischemia groups were established on Wistar rats with 1, 3 and 6 h ischemic times. Intestinal biopsies were collected after laparotomy and at the end of the ischemia periods. DSC measurement was performed on mucosa, on muscular layer and on the total intestinal wall. Our DSC data confirmed that longer warm ischemia period caused more severe damage in the structure of mucosa and muscular layers. According to the results of transition temperature and calorimetric enthalpy suggest that these changes reduced by cold ischemic procedure in University of Wisconsin solution. However, the thermal destruction of each layers following cold preservation injury revealed significant differences compared to normal bowel structure.
Andrea Ferencz, Klra Nedvig, Dnes L?rinczy, Thermochimica Acta, 509 (2010) 50-55
B2614 - DSC analysis of human plasma in breast cancer patients
Breast cancer is the commonest cause of cancer death in women worldwide, and its incidence has been increasing for many years in economically developed countries. Differential scanning calorimetry (DSC) is more and more often applied for oncological purposes, as it could be used as a new method for diagnose and monitoring tumours. In current research liquid plasma components was detected with DSC in breast cancer patients. The study included 19 women with different tumour diameter (0.5Ð75 mm) and with or without regional lymph node metastases (0Ð10 metastatic lymph node). Preoperatively peripheral blood samples were collected from the patients and from healthy controls, and plasma components were analysed by DSC technique. The diameter of the tumour tissue and the number of metastatic lymph nodes were evaluated on the basis of postoperative histological results. In our preliminary study we observed thermic changes (e.g. Tm, calorimetric enthalpy) in the blood plasma of breast cancer patients, and we have found correlation with tumour size and with the degree of regional lymph node invasion as well. Further studies are needed to clarify these relationships, but our application of the DSC method has provided a potential tool for the early diagnosis and monitoring of breast cancer patients.
Istvn Zapf, Tams Fekecs, Andrea Ferencz, Gyrgy Tizedes, Gbor Pavlovics, Endre Klmn, Dnes L?rinczy, Thermochimica Acta, 524 (2011) 88-91
B2613 - DSC examination of kidney tissue following warm ischemia and reperfusion injury
The fact that kidney is sensitive to ischemia/reperfusion (I/R) injury had encouraged us to compare the conventional histology and differential scanning calorimetry (DSC) methods in renal structural changes following experimental warm I/R models. Our histological findings showed that longer warm I/R period caused more severe damage in structure of kidney. The thermal parameters (transition temperature, calorimetric enthalpy) of I/R injury reflected the histological results. These processes correlated with the ischemia time and the degree of injury was more serious after reperfusion periods compared to normal kidney structure, which provides basis for further investigation in different kidney stress models.
Andrea Ferencz, Klra Nedvig, Eszter Lszl--, Tams Magyarlaki, Dnes L?rinczy, Thermochimica Acta, 525 (2011) 161-166
B2612 - DSC examination of intestinal tissue following cold preservation
The fact that small bowel is extremely sensitive to cold preservation had encouraged us to compare the conventional histology and differential scanning calorimetry (DSC) methods in intestinal structural changes following experimental cold storage models. Our histological findings showed that longer cold preservation period caused more severe damage in structure of mucosa and crypts, but there were no changes in the muscular layer. According to our DSC data (transition temperature, calorimetric enthalpy) suggest that the thermal destruction of mucosa, muscular layer and total intestinal wall following preservation injury revealed significant differences compared to normal bowel structure
Andrea Ferencz, Klra Nedvig, Dnes L?rinczy, Thermochimica Acta, 497 (2010) 41-45
B2611 - Whey protein isolateÐchitosan interactions: A calorimetric and spectroscopy study
Isothermal titration calorimetry (ITC) measurements were performed using solutions of whey protein isolate (WPI) and chitosan with different deacetylation degrees (DD), in acetate buffer solutions, pH 3Ð6. Turbidity measurements were performed in parallel in order to follow the changes in aggregation, so as to get deeper insight on the interaction mechanism. The viscosityÐaverage molar mass of chitosan was obtained from intrinsic viscosity measurements, and the interaction enthalpies were derived at the studied pH values. Further, the denaturation process of ?-lactalbumin and ?-lactoglobulin within WPI was characterized by differential scanning calorimetry (DSC). At pH 3, where both chitosan and the proteins are positively charged, a weak carbohydrateÐprotein interaction is observed. When the pH is raised to 6, where the protein charge is expected to be negative, a much stronger interaction takes place. The results are discussed with special emphasis on the effect of pH on the interactions observed in this complex system.
Hilia K.S. de Souza, Guangyue Bai, Maria do Pilar Gonalves, Margarida Bastos, Thermochimica Acta, 495 (2009) 108Ð114
B2610 - Improvement in physicochemical parameters of DPPC liposomes and increase in skin permeation of aciclovir and minoxidil by the addition of cationic polymers
1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) liposomes were prepared by high-pressure homogeniser and coated with two cationic polymers, chitosan (CS) and for the first time Eudragit EPO (EU), respectively. Compared to the control liposomes, the polymeric liposomes showed greater physico-chemical stability in terms of mean particle size and zeta potential at room temperature. In the present study, aciclovir and minoxidil have been used as hydrophilic and hydrophobic candidates. In the presence of the drugs, the polymeric liposomes still showed constant particle size and zeta potential. Influences of polymers and model drugs on thermotropic phase transition of DPPC liposomes were studied by micro-differential scanning calorimetry (microDSC). The influences on configuration of DPPC liposomes were investigated by Fourier transform infrared spectroscopy (FTIR). According to DSC results, cationic poly- mers had a stabilising effect, whereas aciclovir and minoxidil changed the physical properties of the DPPC bilayers by influencing the main phase transition temperature and erasing the pre-transition. The investigation of C@O stretching bands of DPPC at 1736 cmØ1 in FTIR spectra showed that aciclovir has strong hydrogen bonding with C@O groups of DPPC, whereas carbonyl groups were free in minoxidil presence. Moreover, the coating of liposomes with CS or EU led to higher skin diffusion for both drugs. This could be explained as an effect of positively charged liposomes to interact stronger with skin negatively charged surface and their possible interactions with structures below the stratum corneum.
Amra Hasanovic, Caroline Hollick, Kerstin Fischinger, Claudia Valenta, European Journal of Pharmaceutics and Biopharmaceutics 75 (2010) 148Ð153
B2609 - Calf thymus DNAÐmetal ions interactions: Calorimetric and spectroscopic thermal studies
Calorimetric and spectroscopic studies on the interaction of Calf thymus DNA with divalent metal ions have been carried out in order to investigate how different ionic strengths as well as different divalent cations affect the thermal stability of the DNA double helix. The thermodynamic parameters of thermal denaturation of ds-DNA have been determined from solutions containing Mn2+, Co2+, Ni2+, Cu2+, Zn2+ and Cd2+ ions in different concentrations. The results obtained indicated that the nature of the interactions of the metal ions with the DNA molecule depends on metals ion concentration. At low metal concentrations there are no significant changes in the melting temperature value. However at high metal concentrations a decrease in melting temperature was observed, showing that the presence of high divalent cation concentration decreases the double helix stability. Further, it is also shown that the cations tested have significantly different interactions with DNA, even at the same concentration. This reveals clearly that not only the ionic strength is important in DNA stability, but that the changes observed in stability and thermal profile depend largely on the metal used.
Hilia K.S. Souza, Thermochimica Acta, 501 (2010) 1Ð7
B2608 - Vapor pressure, heat capacities, and phase transitions of tetrakis(tertbutoxy) hafnium
Vapor pressure, liquid heat capacities, and phase change enthalpies and temperatures for technologically important organometallic precursor tetrakis(tertbutoxy) hafnium are reported. The vapor pressure was measured using the static method in the temperature range from 280 to 308 K. The standard vaporization enthalpy was derived from the temperature dependence of vapor pressure data. The liquid heat capacities were determined by Calvet calorimetry in the temperature range from 294 to 350 K. The enthalpy and temperature of fusion were measured by differential scanning calorimetry. The present study significantly extends and updates the information on physicochemical properties for this material.
Michal Fulem, Kv?toslav R??i?ka, Fluid Phase Equilibria 311 (2011) 25Ð29
B2607 - Heat capacity, enthalpy and entropy of strontium niobate Sr2Nb2O7 and calcium niobate Ca2Nb2O7
The heat capacity and the enthalpy increments of strontium niobate Sr2Nb2O7 and calcium niobate Ca2Nb2O7 were measured by the relaxation time method (2Ð300 K), DSC (260Ð360 K) and drop calorimetry (720Ð1370 K). Temperature dependencies of the molar heat capacity in the form Cpm = 248.0 + 0.04350T ? 3.948 ? 106/T2 J K?1 mol?1 for Sr2Nb2O7 and Cpm = 257.2 + 0.03621T ? 4.434 ? 106/T2 J K?1 mol?1 for Ca2Nb2O7 were derived by the least-square method from the experimental data. The molar entropies at 298.15 K, Sm¡(298.15 K) = 238.5 ± 1.3 J K?1 mol?1 for Sr2Nb2O7 and Sm¡(298.15 K) = 212.4 ± 1.2 J K?1 mol?1 for Ca2Nb2O7, were evaluated from the low-temperature heat capacity measurements.
J. Leitner, M. Hampl, K. R??i?ka, M. Straka, D. Sedmidubsk?, P. Svoboda, Thermochimica Acta, 475 (2008) 33Ð38
B2606 - Calorimetric investigation of the La2Zr2O7, Nd2Zr2O7, Sm2Zr2O7 and LaYO3 compounds and CALPHAD assessment of the La2O3ÐY2O3 system
Temperature dependence of heat capacity of the La2Zr2O7, Nd2Zr2O7, Sm2Zr2O7 and LaYO3 is measured in the temperature range of 298.15Ð1373 K by differential scanning calorimetry (DSC). The enthalpy of formation from oxides for LaYO3 was measured by high-temperature lead borate solution calorimetry using transposed temperature drop method. Transformations in the LaYO3 were experimentally studied using differential thermal analysis (DTA) up to 2173 K. The obtained results for heat capacity and enthalpy of formation of the LaYO3 and temperatures of transformations were used to re-optimize thermodynamic parameters of the La2O3ÐY2O3 system.
O. Fabrichnaya, M.J. Kriegel, J. Seidel, G. Savinykh, L.P. Ogorodova, I.A. Kiseleva, H.J. Seifert, Thermochimica Acta 526 (2011) 50Ð57
B2605 - Amphiphilic and thermosensitive copolymers based on pullulan and Jeffamine¨:Synthesis, characterization and physicochemical properties
The synthesis of thermosensitive copolymers based on pullulan and polyether amine was performed in water using a watersoluble carbodiimide and Nhydroxysuccinimide as activators. Jeffamine¨ M2005 was chosen as a polyether to impart thermosensitive character to the copolymer. Pullulan was modified into carboxymethylpullulan, to bring carboxylate groups to the polysaccharide so as to further the grafting reaction. The copolymers were characterized by FTIR, 1H NMR spectroscopy and molecular weights measurements (by SEC coupled with MALS/DRI/Viscometer lines). The thermosensitive behaviour of CMPgM2005 copolymers was studied by fluorescence spectroscopy of pyrene, by rheometry and microDSC measurements. The solÐgel transition temperature was found dependent on the solvent, the grafting degree of M2005 and the concentration of the copolymer. For example it was 35 ?C in water, 28 ?C in acid buffer (0.1 M, pH 5.4) and 26 ?C in saline phosphate buffer (0.15 M, pH 7.4) for a grafting degree of 0.20 at a concentration of 5 wt%.
Virginie Dulonga, Georgeta Mocanu, Luc Picton, Didier Le Cerf, Carbohydrate Polymers 87 (2012) 1522Ð 1531
B2604 - Solidification of a PEG 1500-epoxy nanocomposite around a horizontal pipe
A PCM-epoxy phase change material composite (polyethylene glycol 1500-epoxy) was developed as heat storage building element for houses with low energy consumption. The PCM component, polyethylene glycol 1500, was integrated in an epoxy matrix, and presents a phase change interval of 34Ð42 C and an enthalpy of solidification of 103.411 kJ/kg. Experiments on solidification were conducted using a Plexiglas test cell filled with the PEG 1500-epoxy nanocomposite material (P15-E) for further implementation of this material in buildings. The forwarded solidification model assumes negligible convection in the liquid region and predicts the time for radial formation of two regions: a mushy-zone and a solid, annular one around the pipe during solidification. The heat transfer during solidification can be also characterized by the time evolution of both liquid and solid radial fronts. The model was analytically solved using Megerlin approximation concerning ÔÔsolidification with mushy zoneÕÕ, with the third order condition at the external frontier. The experimental values are in agreement with the calculated theoretical curves.
Petrica Mircea Pavel, Mariella Constantinescu, Elena Maria Anghel, Mircea Olteanu, Applied Energy 89 (2012) 482Ð489
B2603 - MicroDSC study of Staphylococcus epidermidis growth
A microcalorimetric study was carried out using a Staphylococcus epidermidis population to determine the reproducibility of bacterial growth and the variability of the results within certain experimental parameters (temperature, bacterial concentration, sample thermal history). Reproducibility tests were performed as series of experiments within the same conditions using either freshly prepared populations or samples kept in cold storage. In both cases, the samples were obtained by serial dilution from a concentrated TSB bacterial inoculum incubated overnight.
Dragos C Zaharia, Cezar Iancu, Alexandru T Steriade, Alexandru A Muntean, Octavian Balint, Vlad T Popa, Mircea I Popa, Miron A Bogdan, BMC Microbiology 2010, 10:322
B2602 - Calorimetric detection of the toxic effect of androgens on fission yeast
Calorimetry is evaluated for study of the toxic effect of environmental androgens on Schizosaccharomyces pombe cells. The results indicate that androstendione, androstandiendione and dehydrotestosterone inhibited S. pombe heat production rate. Although, the turbidimetric method showed that testosterone (TS) had no influence on growth of S. pombe, calorimetry revealed that there was a shift in growth period in samples with TS
Sylwia R--?alska, Bart?omiej Pa?ecz, Jerzy D?ugo?ski, Thermochimica Acta, 474 (2008) 91Ð94
B2601 - Heat Capacities of Some Liquid ?,?-Alkanediols within the Temperature Range between (293.15 and 353.15) K
The heat capacities at constant pressure of 1,2-ethanediol (1,2-EtD), 1,3-propanediol (1,3-PrD), 1,4-butanediol (1,4-BuD), 1,5-pentanediol (1,5-PeD), and 1,7-heptanediol (1,7-HpD) were measured within the temperature range from (293.15 to 353.15) K. The heat capacities of three other compounds, 1,6-hexanediol (1,6-HxD), 1,8-octanediol (1,8-OcD), and 1,9-nonanediol (1,9-NoD), were also measured between their melting point and 353.15 K. All measurements were performed by means of a DSC III (Setaram) differential scanning calorimeter. Assuming that the molar heat capacity shows an additive character, a simple equation was proposed that allows one to describe Cp values of liquid ?,?-alkanediols as a function of temperature and the alkyl chain length with an accuracy below 1 %.
Pawe? Goralski, Mariola Tkaczyk, J. Chem. Eng. Data 2010, 55, 953Ð955
B2600 - Using heat conduction microcalorimetry to study thermal aggregation kinetics of proteins
The thermally induced irreversible aggregation of a monoclonal antibody in different pH buffers was investigated using different techniques such as micro-differential scanning calorimetry (micro-DSC), size exclusion HPLC (SEC) and isothermal microcalorimetry. The kinetics of aggregation of the protein was analyzed in terms of a LumryÐEyring model proceeding via a non-native conformational state. The rate constants and reaction enthalpies of unfolding and consequent aggregation were obtained by fitting the isothermal microcalorimetric and SEC data based on proposed aggregation mechanisms. The consistency of rate constants obtained via isothermal microcalorimetry and SEC indicates it is possible to deconvolute the observed microcalorimetry powerÐtime data obtained from thermally induced protein aggregation.
Donghua (Alan) Zhu, William R. Porter, Michelle A. Long, Wolfgang Fraunhofer, Kenneth M. Gleasona, Yi Gao, Thermochimica Acta, 499 (2010) 1Ð7
B2599 - Measurements of methane hydrate equilibrium in systems inhibited with NaCl and methanol
Natural gas hydrates are ice-like inclusion compounds that form at high pressures and low temperatures in the presence of water and light hydrocarbons. Hydrate formation conditions are favorable in gas and oil pipelines, and their formation threatens gas and oil production. Thermodynamic hydrate inhibitors (THIs) are chemicals (e.g., methanol, monoethylene glycol) deployed in gas pipelines to depress the equilibrium temperature required for hydrate formation. This work presents a novel application of a stepwise differential scanning calorimeter (DSC) measurement to accurately determine the methane hydrate phase boundary in the presence of THIs. The scheme is first validated on a model (ice + salt water) system, and then generalized to measure hydrate equilibrium temperatures for pure systems and 0.035 mass fraction NaCl solutions diluted to 0, 0.05, 0.10, and 0.20 mass fraction methanol. The hydrate equilibrium temperatures are measured at methane pressures from (7.0 to 20.0) MPa. The measured equilibrium temperatures are compared to values computed by the predictive hydrate equilibrium tool CSMGem.
Patrick G. Lafond, Kyle A. Olcott, E. Dendy Sloan, Carolyn A. Koh, Amadeu K. Sum, J. Chem. Thermodynamics 48 (2012) 1Ð6
B2598 - Calorimetric investigation of cyclopentane hydrate formation in an emulsion
Differential scanning calorimetry (DSC) is applied to investigate the formation of cyclopentane hydrates in a water-in-oil emulsion. Protocols of cooling below the ice formation temperature and warming to a temperature above the ice and hydrate melting temperatures are applied. Cyclopentane, which forms hydrates at atmospheric pressure, is a component of the continuous oil phase in the hydrate-forming emulsion and is replaced by iso-octane to obtain a comparable ice-forming emulsion. A method based on comparing the heat flow measured by DSC for samples of identically prepared hydrate-forming and non-hydrate (ice-forming) emulsions is developed to obtain the rate of cyclopentane hydrate growth. Results are reported for a 40% water volume fraction emulsion. Experimental results lead to the conclusion that the hydrate formation takes place primarily at the interface between water drops and the continuous oil phase. In the absence of surfactants, a robust hydrate ÔÔshellÕÕ develops around the water drop limiting transport of hydrate former to the free water which remains trapped inside the hydrate layer. Direct visualization of hydrate formation in larger water drops under the influence of oil-soluble surfactants shows that the hydrate crystals have much smaller features and the appearance is hairy or mushy. A three-step mechanism Ð nucleation, surface growth and radial growth Ð is described to capture the main features of the hydrate formation process. Mechanical stresses developed in the hydrate shell due to volume expansion upon hydrate formation (a liquidÐsolid transition) are analyzed.
Prasad U. Karanjkar, Jae W. Lee, Jeffrey F. Morris, Chemical Engineering Science 64 (2009) 4732 -- 4736
B2597 - Thermodynamic analysis of hydrate-based pre-combustion capture of CO2
This paper presents the phase equilibria of quaternary CO2 + H2 + cyclopentane (CP) + water systems containing gas hydrates and proposes a staged-separation scheme based on the thermodynamic data. The phase equilibria of HLLV of quaternary CO2 + H2 + CP + water systems are determined by using a high-pressure differential scanning calorimeter (DSC). The equilibrium dissociation pressures are dependent on the vapor composition and they shift to lower values with the increase in the CO2 mole fraction. The difference in the dissociation pressures between various CO2 fractions vanishes as the temperature approaches the melting point of CP hydrates at 0.1 MPa. The hydrate dissociation enthalpy is independent of vapor compositions and it is 144 kJmol (gas)?1 over the temperature range. Experimental data are compared to the calculated ones from JohnÐHolder's three-shell model. The prediction matches well with experimental data. The presence of CP reduces the operation pressure of a hydrate-based CO2 capturing process from the pre-combustion stream. Two equilibrium stages of hydrate crystallization and dissociation can enrich CO2 in the vapor-phase significantly from 0.4 of CO2 mole fraction to 0.98 at 282K. This thermodynamic analysis provides a conceptual design for developing a new process of pre-combustion CO2 capture in IGCC plants.
Junshe Zhang, Prasad Yedlapalli, Jae W. Lee, Chemical Engineering Science 64 (2009) 4732 -- 4736
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